{"id":1355,"date":"2025-03-11T19:15:13","date_gmt":"2025-03-11T19:15:13","guid":{"rendered":"https:\/\/cifr.ncsu.edu\/?p=1355"},"modified":"2025-03-13T20:16:46","modified_gmt":"2025-03-13T20:16:46","slug":"timelapse-fluorescence-and-dic-microscopyof-a-crawling-zoospore","status":"publish","type":"post","link":"https:\/\/cifr.ncsu.edu\/index.php\/2025\/03\/11\/timelapse-fluorescence-and-dic-microscopyof-a-crawling-zoospore\/","title":{"rendered":"Timelapse fluorescence and DIC microscopyof a crawling zoospore"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Chytrid fungi make motile spores called zoospores that can crawl like an amoeba or swim with a flagellum. In the Buchler lab, we study a soil chytrid,&nbsp;<em>Spizellomyces punctatus<\/em>, and use Agrobacterium to insert genes that make the fungi fluoresce or glow (Medina et al, eLife 2020). The video shows a glowing zoospore (left = fluorescence image, right = DIC image), recorded at one frame every 2 seconds. The zoospore expresses a Lifeact-tdTomato fusion protein that highlights the actin filaments at the leading edge of the crawling spore. Scale bar is 5 microns. &nbsp;If the zoospore were not confined between a glass slide and a gel pad, it could swim at speeds of up to 100 microns per second using the prominent flagellum visible in the DIC image!<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><em>References<\/em><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Edgar M Medina, Kristyn A Robinson, Kimberly Bellingham-Johnstun, Giuseppe Ianiri, Caroline Laplante, Lillian K Fritz-Laylin, Nicolas E Buchler (2020) Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology eLife 9:e52741<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chytrid fungi make motile spores called zoospores that can crawl like an amoeba or swim with a flagellum. In the Buchler lab, we study a soil chytrid,&nbsp;Spizellomyces punctatus, and use Agrobacterium to insert genes that make the fungi fluoresce or glow (Medina et al, eLife 2020). The video shows a glowing zoospore (left = fluorescence [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1361,"comment_status":"open","ping_status":"closed","sticky":false,"template":"template-1390","format":"standard","meta":{"saved_in_kubio":false,"footnotes":""},"categories":[13],"tags":[],"class_list":["post-1355","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research-highlights"],"_links":{"self":[{"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/posts\/1355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/comments?post=1355"}],"version-history":[{"count":4,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/posts\/1355\/revisions"}],"predecessor-version":[{"id":1362,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/posts\/1355\/revisions\/1362"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/media\/1361"}],"wp:attachment":[{"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/media?parent=1355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/categories?post=1355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cifr.ncsu.edu\/index.php\/wp-json\/wp\/v2\/tags?post=1355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}